PrD-GFP was induced with galactose for 6 hours in the absence or presence of 20 mM auxin. Percentage viability of a strains with different C-terminal aid-tag in MYO2, TPM1/2 and SEC18 and the wild type strain (wt). (C) Quantitative determination of cell viability by Colony-Forming Units (CFU) Assay upon Tpm1/2, Myo2, and Sec18 depletion. An anti-actin antibody was used as loading control. Myo2 was detected with an antibody against an HA-tag present in the aid-degron-tag. (B) Western blot analysis of a Myo2-aid strain (RK1e) without depletion (- Aux) or after auxin-based depletion (+ Aux) for 6 hours. ( A) Wild type (wt), tpm1Δ Tpm2-aid, Myo2-aid and Sec18-aid strains (RK1, RK1c, RK1e, RK1g) were streaked onto SD-ura-leu plates containing 5 mM auxin and incubated for 2 days at 30° C. S2 Fig: Effects of long term depletion of Tpm2, Myo2 and Sec18.
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